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Methods

HPTLC

High-performance thin-layer chromatography - a botanical fingerprinting method used for identity testing.

HPTLC (high-performance thin-layer chromatography) produces a visual "fingerprint" of a botanical's compounds and is widely used for identity testing - confirming a herb is the species it claims to be by matching its band pattern to a reference standard.

It's a workhorse for botanical authentication alongside FTIR and DNA barcoding, and is especially useful for catching adulteration in higher-value botanicals and mushroom extracts.

Guides that cover HPTLC

Cost & Pricing

How Much Does Supplement Testing Actually Cost?

Amazon & Marketplace

Amazon Supplement Compliance: Don't Get Delisted

Getting Started

How to Find a Supplement Testing Lab — The Complete Guide

Getting Started

How to Read a Certificate of Analysis: What the Numbers Actually Mean

Compliance

Importing Supplements: The Testing and Customs Documentation You Need to Clear the FDA

Specialized

Mushroom Supplement Testing: Is It Actually Mushrooms or Just Grain Powder?

Getting Started

How to Test Your Supplement Before Selling (First Batch Checklist)

FDA & GMP

Supplement Finished Product Testing: The GMP Release Checklist

Manufacturing

How to Switch Supplement Testing Labs Without Screwing Up Your Compliance

Free Resources

21 CFR 111 GMP Compliance Checklist — Free PDF Download

Selling Online

Amazon Supplement Approved Lab List — What Labs Amazon Actually Accepts

Selling Online

How to Upload a COA to Amazon Seller Central — Step by Step

Getting Started

Supplement Lab Comparison — Eurofins vs SGS vs Intertek vs ALS vs Independent Labs

Ingredient Testing

Ashwagandha Testing: Withanolides, Root Auth, Heavy Metals

Ingredient Testing

Beetroot Nitrate Testing: Quantifying Active, Label Claims, Metals

Ingredient Testing

Berberine Supplement Testing: HPLC Purity, Adulteration, Identity

Test Methods

Botanical Identity Testing — HPTLC, Microscopy, DNA Barcoding

Test Methods

DNA Barcoding Supplement Identity: Species Authentication by qPCR

FDA & GMP

DSHEA Explained for Supplement Brands

Ingredient Testing

Elderberry Supplement Testing: Anthocyanin Content, Identity, and Microbial Safety

FDA & GMP

FDA Warning Letters for Supplements -- Testing Violations

FDA & GMP

Form 483 Response Guide for Supplement Companies

Ingredient Testing

Ginseng Testing: Ginsenoside Profile by HPLC, Species Authentication, and Pesticide Risks

Test Methods

HPLC Potency Testing for Supplements — How It Works

Getting Started

ISO 17025 for Supplement Testing, Explained

Compliance

Supplement Label Claim Substantiation — Testing Every Word on Your Bottle

Ingredient Testing

Maca Root Testing: Identity, Macamide Markers, and Heavy Metal Screening

Test Methods

Non-GMO PCR Testing for Supplements — How It Works

Getting Started

7 Supplement Tests You're Probably Overpaying For (And How to Fix It)

Ingredient Testing

Pre-Workout Supplement Testing: Banned Stimulants and Label Accuracy

Ingredient Testing

Saw Palmetto Testing: Fatty Acid Profile, Adulteration, Identity

Compliance

Skip Lot Testing for Supplements — When You Can Reduce Testing

Compliance

Structure/Function Claims Testing Requirements

Compliance

Supplement Facts Panel Testing Requirements

Getting Started

Third-Party Supplement Testing: Why It's Non-Negotiable

Ingredient Testing

Turmeric Curcumin Testing: Potency, Lead Chromate, Identity

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